Baculovirus and Insect Cell Gene Expression: Review of Baculovirus Biotechnology
نویسندگان
چکیده
منابع مشابه
Baculovirus and insect cell gene expression: review of baculovirus biotechnology.
The BEVS continues to evolve as a powerful, flexible tool for molecular biology, protein function, and biomedical research. Future developments offer the promise of replacement of hazardous chemical insecticides with environmentally safe biopesticides, construction of baculovirus vectors which encode genes for specific post-translational modifications, and establishment of efficient, stably tra...
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Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
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In the present study we evaluated different systems for the expression of mycobacterial antigen P36 secreted by Mycobacterium bovis. P36 was detected by Western blot using a specific antiserum. The P36 gene was initially expressed in E. coli, under the control of the T7 promoter, but severe proteolysis prevented its purification. We then tried to express P36 in M. smegmatis and insect cells. Fo...
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Kus proteins and many related proteins possess the C-terminal motif C‘ys-AAX. where A is an aliphatic ;imino acid and X is itny iiniino acid. ‘I’his sequence directs ii prenyltransferiise. fh-nesyltransferase in the c;ise of ltas and geranylger;inyItransferase in most other ltas-related proteins. t o prenylate the cysteine residue 11-01. The nitture of the amino acid residue X appears t o deter...
متن کاملcloning and expression of simian rotavirus spike protein (vp4) in insect cells by baculovirus expression system
background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...
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ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1995
ISSN: 0091-6765
DOI: 10.2307/3432869